cd45  (Bioss)

Journal: bioRxiv

Article Title: 4-Methylumbelliferone Restores Age-Related Changes in Perineuronal Nets, Memory and Neuroinflammation

doi: 10.64898/2026.01.30.702721

Figure Lengend Snippet: (A) Representative images of CD45 (red), Iba1 (green) and DAPI (blue) staining in the indicated regions. Arrows mark CD45 + Iba1 − cells and the insets show a magnified view of one such cell. (B) Quantification of CD45 + Iba1 − cells per mm 2 in the indicated areas, showing increased number of PICs in the corpus callosum with aging, which was reduced to young adult levels after 4-MU treatment. Data are presented as mean ± SD and were analyzed using ordinary one-way ANOVA; **p < 0.01, **** p < 0.0001.

Article Snippet: Sections were incubated overnight at 4 °C with primary antibodies against GFAP (chicken, 1:800, ab4674, Abcam), Iba1 (goat, 1:800, 019-19741, Fujifilm), CD45 (rabbit, 1:300, bs-10599R, Bioss), and biotinylated WFA.

Techniques: Staining

Journal: NPJ Regenerative Medicine

Article Title: Deer antler ASCs exosomes ameliorate osteoarthritis via miR-140/MMP13 axis-mediated dual modulation of inflammation and cartilage regeneration

doi: 10.1038/s41536-025-00444-9

Figure Lengend Snippet: A Schematic illustration of the ASCs isolation procedure from deer antler tissue using mechanical dissection and the collagenase digestion method. Created in BioRender. Yuhao, S. (2025) https://BioRender.com/m5easfx . B Representative phase-contrast microscopy image showing the typical spindle-shaped morphology of cultured ASCs at passage 3. Scale bar: 100 μm. C Immunofluorescence analysis of ASC surface markers. ASCs were positive for CD73 and CD90 (green), while negative for CD34 and CD45. Nuclei were counterstained with DAPI (blue). Merged images show co-localization. Scale bar: 100 μm. D – F Multi-lineage differentiation potential of ASCs. D Oil Red O staining showing lipid droplets after 14 days of adipogenic induction. Scale bars: 100 μm. E Alizarin Red S staining demonstrating calcium deposition after 21 days of osteogenic differentiation. Scale bars: 500 μm. F Alcian Blue staining revealing proteoglycan synthesis after 14 days of chondrogenic induction. Scale bars: 100 μm. G Western blot analysis confirming the expression of exosomal markers (CD63, TSG101, and CD9) in isolated ASC-Exos compared to ASCs and culture supernatant. H Representative transmission electron microscopy (TEM) image showing the typical cup-shaped morphology of ASC-Exos with characteristic double-membrane structure. Scale bars: 100 nm. I Nanoparticle tracking analysis (NTA) reveals the size distribution of ASC-Exos with an average diameter of 86.4 nm. J Scatter plot from flow cytometry analysis demonstrating the uniform distribution and homogeneity of the isolated ASC-Exos population.

Article Snippet: For flow cytometric analysis, cells were dissociated using trypsin without EDTA, washed with PBS, and incubated with surface marker antibodies at room temperature for 1 h. The panel included positive markers CD73 (bs-4834R, Bioss, China) and CD90 (bs-0778R, Bioss, China), and negative hematopoietic markers CD45 (bs-4819R, Bioss, China) and CD34 (bs-0646R, Bioss, China), following the manufacturer’s recommended antibody concentrations.

Techniques: Isolation, Dissection, Microscopy, Cell Culture, Immunofluorescence, Staining, Western Blot, Expressing, Transmission Assay, Electron Microscopy, Membrane, Flow Cytometry

Journal: Nature Communications

Article Title: Dual asparagine-depriving nanoparticles against solid tumors

doi: 10.1038/s41467-025-60798-y

Figure Lengend Snippet: a In vivo fluorescence imaging of tumor-bearing mice 24 h after intravenous injection of M Cy5.5 As and M Cy5.5 AHAs ( n = 3 mice). The unit of the scale bar is ×10 9 [p/s/cm 2 /sr]/[µW/cm 2 ]. b Left: Confocal fluorescence imaging of tumor sections after administration of M Cy5.5 As and M Cy5.5 AHAs. The 4T1 tumor cells and immune cells were indicated as CD44 and CD45 positive cells (red fluorescence), respectively. M Cy5.5 As and M Cy5.5 AHAs were pseudo-colored with green fluorescence. DAPI (blue fluorescence) was used to label the nuclei. Scale bars: 15 µm. Right: Values of Pearson’s correlation coefficient between Cy5.5-incorporated NPs and 4T1 tumor cells or immune cells. Data are represented as mean ± SD ( n = 10 independent samples). P values were calculated using an unpaired two-tailed Student’s t test. c Average tumor growth curves. Data are represented as mean ± SD ( n = 6 mice). P values were calculated using a two-way ANOVA followed by Tukey’s multiple comparisons test. d Survival curves of the mice receiving indicated treatment ( n = 6 mice). P values were calculated using a log-rank (Mantel–Cox) test. e The Asn levels in tumor cells isolated from tumors on day 26 after indicated treatment. Data are represented as mean ± SD ( n = 3 independent samples). P values were calculated using a one-way ANOVA followed by Tukey’s post-hoc test. f Heatmap of mRNA levels of ASNS , PSAT1 , GPT2 , MTHFD2 , and SLC1A5 in tumor cells isolated from tumors after indicated treatment. g Left: representative H&E-stained sections of lungs from indicated groups. Metastatic tumors are indicated with black dotted circles. Scale bars: 100 μm. See also Supplementary Fig. for complete data. Right: the number of tumor foci in lungs after indicated treatment. Data are represented as mean ± SD ( n = 3 mice). P values were calculated using a one-way ANOVA followed by Tukey’s post-hoc test. h , i Expression levels of N-cadherin ( h ) and E-cadherin ( i ) in tumors after indicated treatments. Data are represented as mean ± SD ( n = 3 independent samples). P values were calculated using a one-way ANOVA followed by Tukey’s post-hoc test. Asn asparagine, Cy5.5 cyanine 5.5, Rot rotenone, ASNase L -asparaginase. Source data are provided as a Source Data file.

Article Snippet: Tumor sections were stained with either anti-CD44 rabbit polyclonal antibody (Servicebio, GB112054 ) or anti-CD45 rabbit polyclonal antibody (Servicebio, GB113885 ), and visualized using CLSM.

Techniques: In Vivo, Fluorescence, Imaging, Injection, Two Tailed Test, Isolation, Staining, Expressing

Journal: Nature Communications

Article Title: Dual asparagine-depriving nanoparticles against solid tumors

doi: 10.1038/s41467-025-60798-y

Figure Lengend Snippet: a Schematic illustration of the experiment design for post-surgical therapy. Fourteen days after tumor inoculation, primary tumors were removed. Two days later, mice were subjected to various treatments every 3 days for a total of eight doses. On day 75, mice with no sign of tumor relapse and metastasis were challenged with 4T1-Luc cells. b In vivo bioluminescence imaging of the mice after indicated treatment ( n = 6 mice). The unit of the scale bar is ×10 7 p/s/cm 2 /sr. c Tumor growth curves of individual mice after indicated treatment ( n = 6 mice). d Ex vivo bioluminescence images of major organs ( n = 3 mice). The unit of the scale bar is ×10 6 p/s/cm 2 /sr. e Survival curves of the mice receiving the indicated treatment ( n = 6 mice). P values were calculated using a log-rank (Mantel–Cox) test. f In vivo bioluminescence imaging of mice before (day 74) and after (day 75, day 95, and day 105) tumor rechallenge ( n = 5 mice). The unit of the scale bar is ×10 7 p/s/cm 2 /sr. g , h FACS analysis of CD4 + Tcm (gated on CD45 + CD4 + population) ( g ) and CD8 + Tcm cells (gated on CD45 + CD8 + population) ( h ) in splenocytes. Data are represented as mean ± SD ( n = 3 independent samples). P values were calculated using an unpaired two-tailed Student’s t test. Rot rotenone, ASNase L -asparaginase, He heart, Li liver, Sp spleen, Lu lung, Ki kidney, Tcm central memory T. Source data are provided as a Source Data file.

Article Snippet: Tumor sections were stained with either anti-CD44 rabbit polyclonal antibody (Servicebio, GB112054 ) or anti-CD45 rabbit polyclonal antibody (Servicebio, GB113885 ), and visualized using CLSM.

Techniques: In Vivo, Imaging, Ex Vivo, Two Tailed Test